|
|
|
|
|
| Gioria,Ricardo; Braga,Renato de Souza; Krause-Sakate,Renate; Roullier,Caroline; Rosa,Daniel Dias; Moura,Monika Fecury; Souza-Dias,José Alberto Caram de; Sawazaki,Haiko Enok; Camargo,Luis Eduardo Aranha; Rezende,Jorge Alberto Marques. |
| Plants of Capsicum annuum cv. Magali R, resistant to Pepper yellow mosaic virus (PepYMV), which showed severe yellow mosaic, leaf malformation and stunting were observed during the 2003/04 growing season in Lins, São Paulo State, Brazil. Potyvirus-like particles observed in leaf sap from infected plants under the electron microscope reacted with an antiserum against PepYMV in PTA-ELISA. In addition to C. annuum cv. Magali R, this potyvirus also infected systemically the resistant C. annuum cv. Rubia R. The nucleotide sequence of part of the CP gene of this potyvirus shared 96-98% identity with that of other PepYMV isolates. The partial nucleotide sequence of the 3' NTR showed 94-96% identity with that of PepYMV. These data indicate that this potyvirus is a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Potyvirus; RT-PCR; ELISA; Resistance gene. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200017 |
| |
|
|
| Ribeiro,Luiz Fernando Caldeira; Mello,Ana Paula de Oliveira Amaral; Bedendo,Ivan Paulo; Gioria,Ricardo. |
| Begonia is a very appreciated genus of ornamental plants, of economic relevancy, having species of flowers and foliage. In commercial croppings, plants exhibiting characteristic symptoms of phytoplasma infection have been observed, such as shoot proliferation, reduced plant, size small leaves and flowers, and phyllody. Leaves were sampled and total DNA was extracted to be used in nested Polymerase Chain Reaction (PCR), in order to detect and identify an expected phytoplasma. The results confirmed consistently the presence of a phytoplasma associated with symptomatic plants through the amplification of a typical genomic fragment of 1.2 kb by using the universal primers R16mF2/mR1 and R16F2n/R2. The use of specific primers R16(III)F2/R1 allowed to identify... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mollicutes; Yellows; Phytopathogenic prokaryotes. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000500009 |
| |
|
|
| Bonilha,Estela; Gioria,Ricardo; Kobori,Rômulo Fujito; Vecchia,Paulo Tarcísio Della; Piedade,Sônia Maria de Stefano; Rezende,Jorge Alberto Marques. |
| Papaya ringspot virus - type W (PRSV-W) and Zucchini yellow mosaic virus (ZYMV) are the most prevalent viruses in cucurbit crops in Brazil and responsible for frequent yield losses. Diseases caused by these viruses are difficult to control. The objective of this work was to evaluate the effects of mild strains PRSV-W-1 and ZYMV-M on the yield of Cucurbita pepo L. cvs. Samira, Novita Plus, AF 2847, and Yasmin, under plastic greenhouse and field conditions. Plants infected with ZYMV-M and grown in a plastic greenhouse did not exhibit typical leaf symptoms or significant alterations in quantitative and qualitative fruit yield. However, when infected with PRSV-W-1, or PRSV-W-1 + ZYMV-M, the plants exhibited severe leaf mosaic symptoms and reduced fruit... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Control; Potyvirus; Cross protection; Cucurbitaceae. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300019 |
| |
|
|
|
